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oligonucleotides cep 10 spectragreen probe abbott molecular 32 112010 cep 17 spectraorange probe abbott molecular 32 110017  (Abbott Laboratories)
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rna primary probe oligo library  (Twist Bioscience)
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Twist Bioscience rna primary probe oligo library
a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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probe oligos  (Lumiprobe)
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a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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biotinylated dna oligo probes  (Thermo Fisher)
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Thermo Fisher biotinylated dna oligo probes
a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
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oligo probes  (Thermo Fisher)
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Thermo Fisher oligo probes
a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link <t>oligo,</t> then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.
Oligo Probes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link oligo, then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.

Journal: bioRxiv

Article Title: Machine learning-guided spatial omics for tissue-scale discovery of cell-type-specific architectures

doi: 10.64898/2026.02.12.705598

Figure Lengend Snippet: a, Schematic of the two-part labeling workflow. Multiple antibodies are photo-crosslinked to the same oYo-Link oligo, then individually hybridized with distinct barcode probes carrying unique L + R barcode pairs. The barcoded antibody complexes are pooled for multiplex immunostaining. b–f, Distinct protein patterns visualized by cycleHCR in a mouse brain section using antibody complexes conjugated with the same oYo-Link oligo. Antibodies from the same host species were used simultaneously without cross-reactivity (host species indicated for each antibody target in the figure: R, rabbit; M, mouse). Orthogonal 3D rendering performed in Imaris. b, Five-color composite showing CREB-binding protein (CBP), neuron-specific βIII-tubulin (Tuj1), parvalbumin (PV), cannabinoid receptor 1 (CB1), somatostatin receptor 2 (Sstr2), and calbindin in the hippocampal region of a mouse brain section. These targets belong to a subset of 11 antibodies crosslinked to the same oYo-Link sequence (No. 2; ) within the complete 46-protein panel. Scale bar, 100 μm. c, (Left) 3D composite of two nuclear proteins, H4K16ac and MECP2, from the same oYo-Link (No. 2) set. (Right) Corresponding DAPI channel of the same nucleus. Scale bars, 5 μm. d, Three-color composite of SF3a66, AQP4, and VGLUT2 from another oYo-Link (No. 1) set (four antibodies) within the 46-target panel. Scale bar, 500 μm. e,f, Higher-magnification views of boxed regions in (b), showing fine subcellular localization and structural detail. Scale bars (e, f), 20 μm.

Article Snippet: Briefly, the RNA primary probe oligo library (Twist Bioscience) was amplified by PCR using KAPA HiFi HotStart (Roche, KK2502) with primers containing T7 promoter sequences (IDT).

Techniques: Labeling, Multiplex Assay, Immunostaining, Binding Assay, Sequencing

Journal: bioRxiv

Article Title: Machine learning-guided spatial omics for tissue-scale discovery of cell-type-specific architectures

doi: 10.64898/2026.02.12.705598

Figure Lengend Snippet:

Article Snippet: Briefly, the RNA primary probe oligo library (Twist Bioscience) was amplified by PCR using KAPA HiFi HotStart (Roche, KK2502) with primers containing T7 promoter sequences (IDT).

Techniques: Imaging, Sequencing, Multiplex Assay, Expressing, Immunostaining